The masticatory system under varying functional load. Part 2: Effect of reduced masticatory load on the degree and distribution of mineralization in the rabbit mandible.

A reduction in mechanical loading of the mandible brought about by mastication of soft food is assumed to decrease the remodelling rate of bone, which, in turn, might increase the degree of bone mineralization. The effect of a reduction in masticatory functional load on the degree and distribution of mineralization of mandibular bone was investigated in male juvenile New Zealand White rabbits. The experimental animals (n=8) had been raised on a diet of soft pellets from 8 to 20 weeks of age, while the controls (n=8) had been fed pellets of normal hardness. The degree of mineralization of bone (DMB) was assessed at the attachment sites of various jaw muscles, the condylar head, and the alveolar process. Differences between groups and among sites were tested for statistical significance using a Student's t-test and one-way analysis of variance, respectively. The DMB did not differ significantly between the experimental and control animals at any of the sites assessed. However, in the rabbits that had been fed soft pellets, both cortical bone at the attachment sites of the temporalis and digastric muscles and cortical bone in the alveolar process had a significantly higher DMB than cortical bone at the attachment site of the masseter muscle, while there were no significant differences among these sites in the control animals. The results suggest that a moderate reduction in masticatory functional load does not significantly affect the remodelling rate and the DMB in areas of the mandible that are loaded during mastication but might induce a more heterogeneous mineral distribution.

Effect of food consistency on the degree of mineralization in the rat mandible.

A switch to a soft diet, associated with reduced forces applied to the mandible during mastication, may result in an alteration of the degree of mineralization in the mandible. This alteration may be regionally different. The aim of this study was to analyze this alteration by examination of the degree of mineralization in the mandible of growing rats fed with a hard or soft diet. Fifteen Wistar male rats were used in this investigation. After weaning, six rats were fed with a hard diet and the remaining nine rats with a soft diet. After 9 weeks, three-dimensional reconstructions of the cortical and trabecular bone of their mandibles were obtained using a microCT system. The degree of mineralization was determined for the trabecular bone in the condyle and for the cortical bone in the anterior and posterior areas of the mandibular body. In both diet groups the degree of mineralization was significantly (p < 0.01) lower in the trabecular than in the cortical bone. In the mandibular body, the anterior area showed a significantly (p < 0.01) higher degree of mineralization than the posterior area in both diet groups. In both areas the soft diet group had a significantly (p < 0.05 or 0.01) higher degree of mineralization than the hard diet group. The trabecular bone in the condyle of the hard diet group showed a significantly (p < 0.01) higher degree of mineralization than in the soft diet group. The present results indicate the importance of proper masticatory muscle function for craniofacial growth and development.

Daily jaw muscle activity in freely moving rats measured with radio-telemetry.

The jaw muscle activity of rats has been investigated for specific tasks. However, the daily jaw muscle use remains unclear. The purpose of the present study was to examine daily jaw muscle activity, and its variability over time, in the rat (n = 12) by the use of radio-telemetry. A telemetric device was implanted for the continuous recording of masseter muscle and digastric muscle activity. Daily muscle use was characterized by calculating the total time that each muscle was active (duty time), the number of bursts, and the average length of bursts. All parameters were estimated for activities exceeding various levels (5-90%) of the day's peak activity. Daily muscle use remained constant for 4 wk. At the low-activity level, the duty time and burst number of the digastric muscle were significantly (P < 0.01) higher than those of the masseter muscle, whereas the opposite was true at the high-activity level (P < 0.05). No significant intermuscular correlation was observed between the number of bursts of the masseter and digastric muscles, but the interindividual variation of both muscles changed, depending on the level of activation. These findings suggest that the masseter muscle and the digastric muscle show a differential active pattern, depending on the activity level.

Daily number and lengths of activity bursts in rabbit jaw muscles.

Muscle activity has predominantly been studied for specific motor tasks not necessarily representative of normal daily behaviour. The few studies that have examined daily muscle use have quantified this by duty time, merging all levels of muscle activity. Muscle activity can also be characterized by the number, duration and level of bursts. The purpose of this study was to characterize, for various levels of muscle activity, the daily masseter and digastric actions in the rabbit. Characterization was realized by quantification of duty time (summed length of all bursts as a percentage of total time), number of bursts and distribution of burst lengths. A telemetric device was implanted in the two muscles of six rabbits, ensuring the recording of their jaw muscle activities while they moved freely. The continuously transmitted signals over 1 day were analysed. The results showed that (i) more than 100,000 bursts per day exceeded the 2% level of the maximum muscle activity in both muscles, whereas fewer than 100 bursts per day exceeded the 90% level; and (ii) the digastric muscle exhibited a significantly higher duty time than the masseter (respectively, 23% and 14% for activities exceeding the 2% level), which was mainly caused by the on average longer burst lengths at the lowest levels. The characterization of muscle activity in daily burst number and distribution of burst lengths exceeding various activity levels provides valuable information on motor control and enables further investigation of the adaptive capacity of muscles.